2 min readHow Cells Know Which Way to Go
Baltimore, MD — Amoebas aren’t the only cells that crawl: Movement is crucial to development, wound healing and immune response in animals, not to mention cancer metastasis. [Video]
In two new studies from Johns Hopkins, researchers answer long-standing questions about how complex cells sense the chemical trails that show them where to go — and the role of cells’ internal “skeleton” in responding to those cues.
In following these chemical trails, cells steer based on minute differences in concentrations of chemicals between one end of the cell and the other. “Cells can detect differences in concentration as low as 2 percent,” says Dr. Peter Devreotes, director of the Department of Cell Biology at the Johns Hopkins University School of Medicine. “They’re also versatile, detecting small differences whether the background concentration is very high, very low or somewhere in between.”
Working with Dr. Pablo Iglesias, a professor of electrical and computer engineering at Johns Hopkins, Devreotes’ research group members Dr. Chuan-Hsiang Huang, a research associate, and postdoctoral fellow Dr. Ming Tang, devised a system for watching the response of a cellular control centre that directs movement. They then subjected amoebas and human white blood cells to various gradients and analyzed what happened.
“Detecting gradients turns out to be a two-step process,” says Huang. “First, the cell tunes out the background noise, and the side of the cell that is getting less of the chemical signal just stops responding to it. Then, the control centre inside the cell ramps up its response to the message it’s getting from the other side of the cell and starts the cell moving toward that signal.” The results appear on the Nature Communications website on Oct. 27.
But to get going, the cell has to have first arranged its innards so that it’s not just a uniform blob but has a distinct front and back, according to another study from Devreotes’ group. In that work, visiting scientist Dr. Mingjie Wang, and postdoctoral fellow Dr. Yulia Artemenko, tested the role of so-called polarity — differences in sensitivity to chemicals between the front and back of a cell — in responding to a gradient. “In previous studies, researchers added a drug that totally dismantled the cells’ skeleton and therefore eliminated movement. They found that these cells had also lost polarity,” Artemenko says. “We wanted to know whether polarity depended on movement and how polarity itself — independent of the ability to move — helped to detect gradients.”
The team used a pharmaceutical cocktail that, rather than dismantling the cells’ skeleton, froze it in place. Then, as in Huang’s experiments, they watched the response of the cellular control centre to chemical gradients. “Even though the cells couldn’t remodel their skeleton in order to move, they did pick up signals from the gradients, and the response to the gradient was influenced by the frozen skeleton,” Artemenko says. “This doesn’t happen if the skeleton is completely gone, so now we know that the skeleton itself, not its ability to remodel, influences the detection of gradients.” The results appear in the Nov. 6 issue of Cell Reports.
By fleshing out the details of how cells move, the results may ultimately shed light on the many crucial processes that depend on such movement, including development, immune response, wound healing and organ regeneration, and may provide ways to battle cancer metastasis.
Article adapted from a Johns Hopkins Medicine news release.
Publication: The Directional Response of Chemotactic Cells Depends on a Balance between Cytoskeletal Architecture and the External Gradient. Wang, Ming-Jie et al. Cell Reports (2014): Click here to view.