3 min readStudy Identifies Molecular Link Between DNA Damage and Premature Ageing

Philadelphia, PA — Like a beloved pair of jeans, human DNA accumulates damage over time, and older people’s bodies can’t repair it as well.

Many scientists believe a build up of damage can cause cells to enter an irreversible dormant state known as senescence. Cellular senescence is believed to be responsible for some of the telltale signs of ageing, such as weakened bones, less resilient skin and slow-downs in organ function.

DNA damage also seems to play a role in conditions called progerias, which cause premature ageing. Progeria patients have mutations in genes responsible for DNA damage repair. Now researchers from the University of Pennsylvania have pinpointed a molecular link between DNA damage, cellular senescence and premature ageing.

Finding the key players could lead to therapeutic targets for counteracting some of the negative effects of progerias and perhaps even forestalling the effects of natural ageing.

The study, published this week in the journal Cell Reports, took a closer look at the chemical messenger interferon, a molecule that is naturally produced by the body in response to invading pathogens such as viruses. The Penn team found that interferon signalling ramps up in response to double-stranded DNA breaks and that this signalling prompts cells to enter senescence.

When the Penn researchers inactivated the interferon signalling pathways in a mouse model of progeria, however, they were able to extend the animals’ lives. These mice were more fertile, had less gray hair and were larger and more robust than animals in which the interferon pathway was still active.

“Our findings imply that some of the effects of DNA damage could be managed by perhaps locally blocking interferon signalling,” said Serge Y. Fuchs, senior author on the study and a professor of cell biology in Penn’s School of Veterinary Medicine.

Collaborators on the study included Christopher J. Lengner, an assistant professor at Penn Vet, and Roger A. Greenberg and F. Brad Johnson, associate professors at Penn’s Perelman School of Medicine. The lead authors were Qiujing Yu and Yuliya V. Katlinskaya of Penn Vet.

Though the main role of interferon is to help cells respond to pathogens, Fuchs and his colleagues began considering a growing body of evidence linking interferon to DNA damage. Previous studies had shown that people with autoimmune diseases characterized by up-regulated interferon signalling also had a build-up of DNA strand breaks. And cancer treatments such as chemotherapy and radiation, which cause DNA damage, also lead to increased interferon signalling.

“It is known that if you add a DNA-damaging agent in a high enough concentration, you will trigger interferon production,” said Fuchs, who is also director of the Mari Lowe Center for Comparative Oncology at Penn Vet. “But we didn’t know whether it was the DNA damage itself that led to the interferon induction or if some other aspect of the cells being killed led to the induction.”

The Penn team first examined the effect of DNA damage on interferon signalling. Using an experimental technique that Roger Greenberg and his lab had earlier developed that creates double-stranded breaks in DNA without chemotherapies or radiation, the researchers found that levels of the interferon protein IFNβ and a reporter molecule tied to interferon production, IRF7, both rose.

The Penn team also found significantly higher levels of IFNβ expression in the older cells from human patients with progerias and also in mice with a mutation that causes a progeria-like condition. Using a drug that blocks IFNβ caused fewer cells to enter senescence, suggesting that senescence was triggered by DNA-damage-induced interferon signalling.

One of the reasons senescence is believed to lead to the characteristic changes of ageing is that it affects stem cells, which normally serve to replenish populations of healthy cells. Earlier studies had shown that mice lacking the Terc gene, which is key to DNA repair, have lower stem cell function and age prematurely, losing fertility and developing scaly skin, grey fur and shrunken, hunched bodies. These mice also have abnormalities in their intestinal tissues, a site known to be greatly affected by stem cell failure.

As a final, in vivo test, the researchers bred Terc-deficient mice to animals also lacking an interferon receptor. These animals had reduced signs of premature ageing; they were more fertile, had less grey hair, were larger and lived longer on average than mice lacking only Terc.

“We could rescue the majority of these phenotypes by abolishing interferon signalling,” Fuchs said, “showing that there is a substantial role of interferon in ageing that is caused by persistent DNA damage.”

For people who suffer from the effects of accelerated ageing after undergoing treatments such as radiation that damage DNA or who suffer from acute radiation poisoning these findings hint at novel therapies.

“Perhaps their problems could be alleviated if we neutralized interferon locally,” said Fuchs.

While the current study doesn’t pertain directly to normal ageing in healthy individuals, Fuchs says future studies could shed light on ways to mitigate its negative effects.

“Since natural ageing is connected with the DNA damage we accumulate over our lifetime and with decline in the stem cell functions, our skin is not repairing as well, our bones are not holding as well as they used to. There is rationale for the future studies on the role of interferon in normal ageing.”

More generally, Fuchs said, the findings highlight interferon as a sort of “guardian of the genome.”

“It works at different levels: For a single cell, by protecting it from viruses that can change its genetic information; for a multi-cellular organism, by preventing genetically altered cells from proliferating via senescence; and for a society, by decreasing fertility of individuals carrying damaged genome.”

Article adapted from a University of Pennsylvania news release.

Publication: DNA-Damage-Induced Type I Interferon Promotes Senescence and Inhibits Stem Cell Function. Yu, Qiujing et al. Cell Reports (2015): Click here to view.

Functional Genomics

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