3 min readResearch Finds Tumour Immune Fitness Determines Survival of Lung Cancer Patients
La Jolla, CA — In recent years, immunotherapy, a new form of cancer therapy that rouses the immune system to attack tumour cells, has captivated the public’s imagination. When it works, the results are breathtaking. But more often than not it doesn’t, and scientists still don’t know why.
Publishing in the June 19, 2017, issue of Nature Immunology, researchers at La Jolla Institute for Allergy and Immunology, identify a subpopulation of T cells in tumours known as tissue-resident memory T cells (TRM) as an important distinguishing factor between cancer patients whose immune system mounts an effective anti-tumour response and those who are unable to do so. Their finding emerged from the first large-scale effort to profile the gene expression patterns of cytotoxic T cells isolated directly from patients’ tumours.
“Systematically studying cancer patients’ immune cells reveals a lot of information,” says LJI Associate Professor and William K. Bowes Jr. Distinguished Professor Dr. Pandurangan Vijayanand, who co-directed the study with Professor Christian Ottensmeier at the University of Southampton, England. “It could be a baseline test to predict whether a patient will respond to immunotherapy and guide the choice of immunotherapy that is most likely to be effective. It is almost like judging tumour immune fitness,” adds Vijayanand. The systematic profiling of tumour-infiltrating T cells will also provide new insight into their basic biology revealing new potential immunotherapy drug targets.
Scientists initially found that when T cells were swarming a patient’s tumour that patient lived longer. Over time, however, they found that T-cells lose their fervour and cancer cells gain the upper hand. In the last decade they discovered why: Inhibitory molecular signals emitted from a tumour or its environment undercut the immune response, making tumour cells invisible to the immune system. One class of cancer immunotherapy drugs, known as checkpoint blockade inhibitors, disables either PD-1 or CTLA-4, two known molecules that allow cancer cells to live and multiply undetected by the immune system.
“The challenge with immunotherapy based on PD-1 and CTLA-4 is that if they work, they work miraculously, but they only work in about 30 percent of patients,” says the study’s first author, Dr. Anusha-Preethi Ganesan, a physician in the Division of Pediatric Hematology and Oncology at Rady’s Children’s Hospital, UC San Diego. “If we are doing all these immunotherapies based on activating T cells to kill tumour cells it is really important to know what the transcriptional profiles of these T cells are, what molecules do they make?”
To uncover the underlying reasons why some patients see little or no benefit and to identify those patients most likely to respond, Ganesan utilized advanced genomics tools to define the molecular features of a robust anti-tumour immune response using freshly resected tumours from patients with cancer. Comparing gene expression profiles of cytotoxic T cells (CTLs) isolated from 41 head and neck tumours and 36 untreated, early stage lung tumours with CTLs isolated from adjacent normal lung tissue, Ganesan identified a shared molecular fingerprint between different tumour types suggesting extensive reprogramming of CTLs infiltrating tumour tissue.
Beyond their shared molecular signature, tumor-infiltrating CTLs differed widely in their expression of molecules associated with T cell activation and known immune checkpoints. “There is a huge deal of heterogeneity, which has a lot of implications for immunotherapy,” says Ganesan. “We see the traditional immunotherapy targets but they are not expressed in every single patient, which means not every patient is a candidate for currently available immunotherapies targeted at PD-1 or CTL4-1. That’s why having the full transcriptional profile is so important to understand the entire complexity of the immune network and to identify novel targets.”
Interestingly, gene expression patterns that signal the presence of tissue resident memory T cells (TRM) corresponded with better anti-tumour activity. The only recently identified tissue resident memory T cells act as local first responders that provide rapid onsite immune protection. A large scale analysis in an independent cohort of 689 lung cancer patients confirmed that patients with a high density of TRM cells in tumour tissue survived significantly longer, demonstrating that these cells serve a critical role in protecting against tumour recurrence.
“Any time you remove a tumour, the patient is a ticking time bomb after that. In some people it will come back and it others it won’t,” says Vijayanand. “Our study suggests that the presence of these tissue resident memory cells is an important factor in determining whether somebody is having an effective immune response against cancer and whether they will live longer.”
Article adapted from a La Jolla Institute for Allergy and Immunology news release.
Publication: Tissue-resident memory features are linked to the magnitude of cytotoxic T cell response in human lung cancer. Anusha-Preethi Ganesan et al. Nature Immunology (2017): Click here to view.