3 min readSystems Biology Reveals Babies’ Detailed Developmental Trajectory During Week 1 of Life
Boston, MA — The first week of a newborn’s life is a time of rapid biological change as the baby adapts to living outside the womb, suddenly exposed to new bacteria and viruses. Yet surprisingly little is known about these early changes. An international research study co-led by Boston Children’s Hospital has pioneered a technique to get huge amounts of data from a tiny amount of newborn blood, creating the most detailed accounting to date.
The work, published March 12 in Nature Communications, profiled molecular changes in the first week of newborn life, including what genes are turned on, what proteins are being made and what metabolites are changing. It establishes a common developmental pathway for the first week of a newborn’s life, providing a baseline to further our understanding of newborn health and, in particular, the impacts of vaccines on newborns.
“Most infections in the world occur early in life, and newborns have the greatest susceptibility and the worst outcomes,” says Dr. Ofer Levy, director of the Precision Vaccines Program at Boston Children’s Hospital and a senior author on the paper. “This work provides a valuable window into health and disease in the first week of life.”
The research team includes members of the Expanded Program on Immunization Consortium (EPIC), an international collaboration to employ a systems biology approach to understanding vaccine action in early life. Levy partnered with Dr. Beate Kampmann, of the London School of Hygiene & Tropical Medicine (LSHTM) and the Medical Research Council (MRC)- The Gambia, and Dr. Tobias Kollmann, University of British Columbia (UBC) to establish the consortium, which has its administrative hub in the Precision Vaccines Program.
Tiny sample, big knowledge
Previous efforts to gather data on newborn development have been limited by the challenge of obtaining a large enough blood sample from a tiny newborn. The team overcame this challenge with new techniques, partnering with Dr. Robert Hancock, of UBC, who led an RNA analysis, and Dr. Hanno Steen, of Boston Children’s, who led a proteomics analysis. Using sophisticated software to analyze complex data derived from less than half a teaspoon of blood, the UBC labs of Hancock and Dr. Scott Tebbutt, found thousands of changes over the first week, including changes in gene expression and components of immune defence such as interferons, neutrophil function and complement pathways.
“We demonstrated that it’s possible to recruit newborns in a resource-poor setting, obtain small amounts of their blood, process it, ship it, conduct systems biology assays and integrate the results — turning big data into knowledge,” says Levy. “Key to our analysis was comparing each newborn at day 1, day 3 and day 7 to their own baseline condition on the day of birth. That’s when we discovered dramatic molecular changes driven by development.”
An inter-continental effort
The researchers piloted their systems biology approach with a group of infants from The Gambia in West Africa after first obtaining permission from village elders and informed consent from mothers in local languages such as Mandinka, Fula and Wolof. They then validated their approach with a second group of Australasian newborns recruited by Dr. William Pomat, of the Papua New Guinea Institute of Medical Research and Dr. Anita van den Biggelaar, of the University of Western Australia.
The two independent cohorts showed a common, highly dynamic developmental trajectory. The findings suggest that the changes do not occur at random, but instead follow an age-specific pathway.
“This common trajectory is exciting as it allows us to ask bigger questions about the differences between different populations and the impact of biomedical interventions such as vaccines on development,” says Levy.
Optimizing vaccines in early life
The study establishes a baseline for health and disease in early life that can help measure responses to key medical interventions and the impact of factors such as diet, disease and maternal health. Levy is particularly interested in studying the impact of immunization. Newborns’ responses to immunization are distinct from those of older individuals, and much needs to be learned to optimize the use and benefit of vaccines in early life.
“Currently, most vaccines are developed by trial and error,” says Levy. “We seek deep molecular insight into vaccine function in early life so we can better develop infant vaccines for the future.”
Article adapted from a Boston Children’s Hospital news release.
Publication: Dynamic molecular changes during the first week of human life follow a robust developmental trajectory. Amy H. Lee et al. Nature Communications (2019): Click here to view.