4 min readPutting Functional Proteins in Their Place
Upton, NY — Scientists have organized proteins–nature’s most versatile building blocks–in desired 2-D and 3-D ordered arrays while maintaining their structural stability and biological activity. They built these designer functional protein arrays by using DNA as a programmable construction material. The team–representing the U.S. Department of Energy’s (DOE) Brookhaven National Laboratory, Columbia University, DOE’s Lawrence Berkeley National Laboratory, and City University of New York (CUNY)–described their approach in the June 17 issue of Nature Communications.
“For decades, scientists have dreamed about rationally assembling proteins into specific organizations with preserved protein function,” said corresponding author Oleg Gang, leader of the Center for Functional Nanomaterials (CFN) Soft and Bio Nanomaterials Group at Brookhaven Lab and a professor of chemical engineering and of applied physics and materials science at Columbia Engineering. “Our DNA-based platform has enormous potential not only for structural biology but also for various bioengineering, biomedical, and bionanomaterial applications.”
The primary motivation of this work was to establish a rational way to organize proteins into designed 2-D and 3-D architectures while preserving their function. The importance of organizing proteins is well known in the field of protein crystallography. For this technique, proteins are taken from their native solution-based environments and condensed to form an orderly arrangement of atoms (crystalline structure), which can then be structurally characterized. However, because of their flexibility and aggregation properties, many proteins are difficult to crystallize, requiring trial and error. The structure and function of proteins may change during the crystallization process, and they may become nonfunctional when crystallized by traditional methods. This new approach opens many possibilities for creating engineered biomaterials, beyond the goals of structural biology.
“The ability to make biologically active protein lattices is relevant to many applications, including tissue engineering, multi-enzyme systems for biochemical reactions, large-scale profiling of proteins for precision medicine, and synthetic biology,” added first author Shih-Ting (Christine) Wang, a postdoc in the CFN Soft and Bio Nanomaterials Group.
Though DNA is best known for its role in storing our genetic information, the very same base-pairing processes used for this storage can be leveraged to construct desired nanostructures. A single strand of DNA is made of subunits, or nucleotides, of which there are four kinds (known by the letters A, C, T, and G). Each nucleotide has a complementary nucleotide it attracts and binds to (A with T and C with G) when two DNA strands are near each other. Using this concept in the technique of DNA origami, scientists mix multiple short strands of synthetic DNA with a single long strand of DNA. The short strands bind to and “fold” the long strand into a particular shape based on the sequence of bases, which scientists can specify.
In this case, the scientists created octahedral-shaped DNA origami. Inside these cage-like frameworks, they placed DNA strands with a particular “colour,” or coding sequence, at targeted locations (center and off center). To the surface of proteins–specifically, ferritin, which stores and releases iron, and apoferritin, its iron-free counterpart–they attached complementary DNA strands. By mixing the DNA cages and conjugated proteins and heating up the mixture to promote the reaction, the proteins went to the internal designated locations. They also created empty cages, without any protein inside.
To connect these nanoscale building blocks, or protein “voxels” (DNA cages with encapsulated proteins), in desired 2-D and 3-D arrays, second author and Columbia PhD student Brian Minevich designed different colors for the external bonds of the voxels. With this colour scheme, the voxels would recognize each other in programmable, controllable ways leading to the formation of specifically prescribed types of protein lattices. To demonstrate the versatility of the platform, the team constructed single- and double-layered 2D arrays, as well as 3D arrays.
“By arranging the colours in a particular way, we can program the formation of different lattices,” explained Gang. “We have full control to design and build the protein lattice architectures we want.”
To confirm that the proteins had been encapsulated inside the cages and the lattices had been constructed as designed, the team turned to various electron- and x-ray-based imaging and scattering techniques. These techniques included electron microscopy (EM) imaging at the CFN; small-angle x-ray scattering at the National Synchrotron Light Source II (NSLS-II) Complex Materials Scattering (CMS) and Life Science X-ray Scattering (LiX) beamlines at Brookhaven; and cryogenic-EM imaging at the Molecular Foundry (MF) of Lawrence Berkeley and the CUNY Advanced Science Research Center. The CFN, NSLS-II, and MF are all DOE Office of Science User Facilities; CFN and MF are two of five DOE Nanoscale Science Research Centers.
“The science was enabled by advanced synthesis and characterization capabilities at three user facilities within the national lab system and one university-based facility,” said Gang. “Without these facilities and the expertise of scientists from each of them, this study wouldn’t have been possible.”
Following these assembly studies, they investigated the biological activity of ferritin. By adding a reducing reagent to the ferritin lattice, they induced the release of iron ions from the center of the ferritin proteins.
“By monitoring the evolution of SAXS patterns during iron release, we could quantify how much iron was released and how quickly it was released, as well as confirm that the integrity of the lattice was maintained during this protein operation,” said Minevich. “According to our TEM studies, the proteins remained inside the frames.”
“We showed that the proteins can perform the same function as they do in a biological environment while keeping the spatial organization we created,” explained Wang.
Next, the team will apply their DNA-based platform to other types of proteins, with the goal of building more complex, operational protein systems.
“This research represents an important step in bringing together different components from real biological machinery and organizing them into desired 2-D and 3-D architectures to create engineered and bioactive materials,” said Gang. “It’s exciting because we see the rational path for fabricating desired functional bio-nano systems never-before produced by nature.”
Article adapted from a Brookhaven National Laboratory news release.
Publication: Designed and biologically active protein lattices. Wang, ST et al. Nature Communications (June 17, 2021): Click here to view.