1 min readScientists Discover Genetic Differences Between ‘Identical’ Twins
Ebersberg, Germany – A multidisciplinary Eurofins team in the Eurofins Genomics laboratory in Ebersberg, Germany, has announced the successful completion of a research project to genetically discriminate “identical” monozygotic twins.
So far there have been only theoretical considerations against the experimental finding and dogma that monozygotic twins are genetically fully identical. Statistically, around 6 of 1,000 males are identical twins. Up to now, forensic DNA fingerprinting testing could not be used in crime or paternity cases involving identical twins, as there was no possibility of genetically discriminating between them. Such cases are regularly discussed in the World’s press, including murder, child custody and heritage cases.
Forensic laboratories around the world had accepted these analytical restrictions, but Eurofins scientists wanted to push these limits of DNA testing. They used the unique combination of forensics and genomics labs available at Eurofins to reach this milestone.
Technically, the Eurofins scientists applied Eurofins’ ultra-deep next generation sequencing and associated bioinformatics techniques. They sequenced DNA from sperm samples of two twins and from a blood sample of the child of one twin. Bioinformatics analysis revealed five mutations, so called Single Nucleotide Polymorphisms (SNPs) present in the twin father and the child, but not in the twin uncle. The SNPs were confirmed by classical Sanger sequencing. The results give experimental evidence for the hypothesis that rare mutations will occur early after or before the human blastocyst has split into two, the origin of twins, and that such mutations will be carried on into somatic tissue and the germ line.
The genetic differences found and the method used provide a solution to solve forensic and paternity cases involving monozygotic twins as originator of DNA traces in crime, or as alleged fathers.
Publication: Finding the needle in the haystack: Differentiating “identical” twins in paternity testing and forensics by ultra-deep next generation sequencing. Jacqueline Weber-Lehmann et al. Forensic Science International: Genetics, November (2013): http://dx.doi.org/10.1016/j.fsigen.2013.10.015